- SynonymILT1,LIR7,CD85H,LIR-7,LILRA2,ILT1CD85H,CD85h,ILT1,ILT-1,ILT1CD85H,CD85h antigen,CD85 antigen-like family member H
- SourceBiotinylated Human LILRA2, His,Avitag (LI2-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Asn 449 (Accession # Q8N149-1).Predicted N-terminus: Gly 24Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 50.7 kDa. The protein migrates as 67-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LILRA2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA2, His,Avitag (Cat. No. LI2-H82E9) with a linear range of 0.625-5 μg/mL (QC tested).
- BackgroundLILRA2 (Leukocyte immunoglobulin like receptor A2), also known as CD85H and LIR7 (Leukocyte immunoglobulin-like receptor 7). It belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail.LILRA2 contains 4 Ig-like C2 type domains in the extracellular region. LILRA2 does not bind class I MHC antigens. LILRA2 is expressed predominantly on monocytes and B cells, and at lower levels on dendritic cells and natural killer cells. LILRA2 is an activating receptor that inhibits dendritic cell differentiation and antigen presentation and suppresses innate immune response.
- References
- (1)Hirayasu K., et al., 2016, Nat Microbiol.,1(6), 16054.
- (2)Brown D., et al., 2004, Tissue Antigens.,64(3), 215-25.
- (3)MacGlashan DW Jr1., et al.,2008, J Immunol., 180(6), 4208-17.
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