- SynonymIL-17C,Cytokine CX2,IL17C
- SourceHuman IL-17C, His Tag (ILC-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 19 - Val 197 (Accession # Q9P0M4-1).Predicted N-terminus: His 19Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.6 kDa. The protein migrates as 24-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IL-17C, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-17C, His Tag (Cat. No. ILC-H52H7) at 2 μg/mL (100 μL/well) can bind Human IL-17 RE (155-454), Fc Tag (Cat. No. ILE-H5256) with a linear range of 0.2-8 ng/mL (QC tested).
Loaded Human IL-17 RE (155-454), Fc Tag (Cat. No. ILE-H5256) on Protein A Biosensor, can bind Human IL-17C, His Tag (Cat. No. ILC-H52H7) with an affinity constant of 0.714 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- BackgroundInterleukin-17C(IL-17C) is a glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity, binds to a heterodimer formed by IL17RA and IL17RE. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Synergy is also observed with TNF and IL1B in inducing DEFB2 from keratinocytes.
- References
- (1)Ramirez-Carrozzi V, et al. 2011. Nat Immunol. 12(12):1159-66.
- (2)Chang SH, et al. 2011 Oct 28. Immunity. 35(4):611-21.
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