- SynonymIGFBP1,PP12,IBP1
- SourceRhesus macaque IGFBP-1, His Tag (IG1-C52H1) is expressed from human 293 cells (HEK293). It contains AA Ala 48 - Asn 281 (Accession # XP_001085935.2).Predicted N-terminus: Ala 48Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.2 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>98% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Rhesus macaque IGFBP-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
Immobilized Human IGF-I, Fc Tag (Cat. No. IG1-H4269) at 5 μg/mL (100 μL/well) can bind Rhesus macaque IGFBP-1, His Tag (Cat. No. IG1-C52H1) with a linear range of 2-31 ng/mL (QC tested).
- BackgroundInsulin-like growth factor-binding protein 1 (IGFBP1) is also known as placental protein 12 (PP12), which contains oneIGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP1 can bindd both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of IGFBP1 can prolongd the half-life of the IGFs and alterd their interaction with cell surface receptors. Furthermore, IGFBP1 can promote cell migration. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
- References
- (1)Lee Y.-L., et al., 1988, Mol. Endocrinol. 2:404-411.
- (2)Jones J.I., et al., 1993, J. Biol. Chem. 268:1125-1131.
- (3)Sala A., et al., 2005, J. Biol. Chem. 280:29812-29819.
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