- SynonymGALE,UDP-glucose 4-epimerase,Galactowaldenase
- SourceHuman Galactowaldenase, His Tag (GAE-H5142) is expressed from E.coli cells. It contains AA Ala 2 - Ala 348 (Accession # AAH01273).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 39.1 kDa. The protein migrates as 39 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Galactowaldenase, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundUDP-glucose 4-epimerase, a member of the NAD(P)-dependent epimerase (dehydratase) family, is also known as galactowaldenase (GALE), UDP-N-acetylgalactosamine 4-epimerase (UDP-GalNAc 4-epimerase) and UDP-N-acetylglucosamine 4-epimerase (UDP-GlcNAc 4-epimerase),which is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. GALE can catalyze two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and GALE (UDP-GalNAc) when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
- References
- (1)Schulz J.M., et al., 2004, J. Biol. Chem. 279:32796-32803.
- (2)Thoden J.B., et al, 2001, J. Biol. Chem. 276:15131-15136.
- (3)Holden HM., et al., 2003, J. Biol. Chem. 278 (45): 43885–8.
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