- SynonymFcRn,FCGRT & B2M
- SourceHuman FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR& BLI verified) (FCM-H5286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1(FCGRT) & P61769-1(B2M)).Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)Request for sequence
- Molecular Characterization
Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR& BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR& BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR& BLI verified) (Cat. No. FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herceptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200) (QC tested).
Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR& BLI verified) (Cat. No. FCM-H5286) on CM5 Chip, can bind Herceptin with an affinity constant of 1.19 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR& BLI verified) (Cat. No. FCM-H5286) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.27 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) on AR2G Biosensor, can bind Herceptin with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human Serum Albumin, His,Avitag (Cat. No. HSA-H82E3) on SA Biosensor, can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) with an affinity constant of 0.641 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Citations
Enhancing durability of CIS43 monoclonal antibody by Fc mutation or AAV delivery for malaria prevention
Authors: Kielau Kisalu, Neville et al.
Journal: JCI Insight 2020
Application: BLI
Request for Full-text
Engineered antibody Fc variants for enhanced serum half life
Authors: G Georgiou, et al
Journal: US20190048078A1 2019
Application: SPR
Request for Full-text
Control of therapeutic IgG antibodies galactosylation during cultivation process and its impact on IgG1/FcγR interaction and ADCC activity
Authors: Simonov V, et al
Journal: Biologicals 2019
Application: BLI
Request for Full-text
Comparative studies of the serum half-life extension of a protein via site-specific conjugation to a species-matched or -mismatched albumin.
Authors: Yang B, et al.
Journal: Biomater Sci 2018
Application: ELISA
Request for Full-text
Unidirectional transport of IgG by neonatal Fc receptor in human thyrocytes varies across different IgG subclasses
Authors: Zhao C, et al.
Journal: Mol Cell Endocrinol 2018
Application: SPR
Request for Full-text
Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody
Authors: Kenniston JA, et al.
Journal: J Biol Chem 2017
Application: ELISA & SPR
Request for Full-text
- BackgroundFCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
- References
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