- SynonymFCGR3A,CD16A,FCG3,FCGR3,IGFR3
- SourceHuman CD16a (V176), His Tag (SPR& BLI verified) (CD8-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).Predicted N-terminus: Gly 17Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by reduced SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD16a (V176), His Tag (SPR& BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
ImmobilizedHuman CD16a (V176), His Tag (SPR& BLI verified) (Cat. No.CD8-H52H4) on CM5 Chip via anti-His antibody, can bindHerceptinwith anaffinity constant of 163 nM as determined in SPR assay (BiacoreT200) (QC tested).
Loaded Human CD16a (V176), His Tag (SPR& BLI verified) (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (V176), His Tag (SPR & BLI verified) (Cat. No. CD8-H52H4) with an affinity constant of 0.37 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (V176), His Tag (SPR & BLI verified) (Cat. No. CD8-H52H4) with an affinity constant of 0.31 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Citations
Charge variant analysis of proposed biosimilar to Trastuzumab
Authors: Dakshinamurthy P, et al.
Journal: Biologicals 2017
Application: SPR
Request for Full-text
- BackgroundCD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibodydependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
- References
(1)Webster NL., et al., 2006, J Leukoc Biol. 79: 294-302.
(2)Nagarajan S., et al., 1995, J Biol Chem. 270: 25762-70.
(3)Ravetch JV., 1989, J.Exp.Med.170:481.
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