- SynonymB3GAT3,GlcAT-I,GlcUAT-I
- SourceHuman B3GAT3, His Tag (B33-H5141) is expressed from E.coli cells. It contains AA Glu 72 - Val 335 (Accession # AAH07906).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 30.2 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>92% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human B3GAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
- BackgroundGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3), a member of the glycosyltransferase 43 family, is also known as beta-1,3-glucuronyltransferase 3, glucuronosyltransferase I (GlcAT-I) and UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase (GlcUAT-I). B3GAT3 plays a role in glycosaminoglycans biosynthesis and be involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. B3GAT3 can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Furthermore,B3GAT3 shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.
- References
- (1)Kitagawa H., et al., 1998, J. Biol. Chem. 273:6615-6618.
- (2)Pedersen L.C., et al., 2000, J. Biol. Chem. 275:34580-34585.
- (3)Tone Y., et al., 2008, J. Biol. Chem. 283:16801-16807.
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