BaseScope™ Reagent Kit v2 - RED Assay is based on ACD"s patented signal amplification and background suppression technology. This high sensitive RNA ISH technique and can be used for short targets and splice variants. The BaseScope™ Reagent Kit v2- RED assay includes the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is expected ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield. Microscope .Each kit contains the BaseScope Detection Kit v2 (Cat# 323910), RNAscopeﾮ Pretreat Reagents-H202 and Protease Reagents (Cat#322381), RNAscope Target Retrieval (Cat#322000), RNAscope Wash Buffer (Cat#310091).