miRNAscope™ HD Reagent Kit RED Assay is based on ACD"s patented signal amplification and background suppression technology. This is a highly sensitive RNA ISH technique and can be used for small RNAs including ASOs, miRNAs, and siRNAs. The miRNAscope™ HD Reagent Kit ? RED assay includes the Fast Red dye which offers a higher contrast and is the first choice in situ hybridization application where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is expected ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope. Each kit contains the miRNAscope™ HD Detection Reagents - RED (Cat# 324510), RNAscopeﾮ Pretreat Reagents-H202 and Protease Reagents (Cat#322381), RNAscope Target Retrieval (Cat#322000), and RNAscope Wash Buffer (Cat#310091).