Details
Description
- VividViolet™isaneutralcarrierwhichenhancestherecoveryofnucleicacidswhenaddedpriortoethanolprecipitation
- Eliminatestheneedforlowincubationtemperature
- FunctionssimilarlytoRNAorglycogentoimproverecoverywhenprecipitatingdilutesolutionsofnucleicacids,buthastheadvantageofaddingnoBIOLOGicalmaterial
- Chemicallysynthesized;willnotinterfereinsubsequentmolecularbiologymanipulations
- ResultsinahighlyvisIBLepurplepelletaftercentrifugationoftheprecipitationmixtureandthuseliminateslossofnucleicacidpelletsduringethanolwashstepssincethenucleicacidsarereADIlyseen
- Avoidsthenecessityofbeingremovedpriortonextmolecularbiologystep
- Helpstodeterminewhennucleicacidsarethoroughlydissolved
- DoesnotinterferewithSDA,PCR,RT-PCR,restrictionenzymedigestion,gelelectrophoresisorfluorometricdeterminationofnucleicacidcontent
- Doesnotprecipitatenucleicacidsshorterthan20basepairs,unincorporatednucleotidesandshortprimersfromPCRproductscanberemoved
- VividVioletdoesabsorbatA260andthereforeacorrectionoftheabsorbancereadingisrequired
Concentration
10.0mg/ml
QualityControl
Allpreparationsareassayedforcontaminatingendonuclease,exonuclease,nonspecificRNaseandsingle-anddouble-strandedDNaseactivities
Notes
- Sufficientfor100x50μLprecipitations
- Suppliedat10mg/ml
- Storedinnuclease-freewater
Procedure
SeeCertificateofAnalysisBelow.
StorageConditions
Storeat+4°C
Shippedatroomtemperature
Downloads
CertificatesofAnalysis-
Lot270736PDF
MSDS-
MSDSPDF
Categories
SpecialtyReagents