Description

Simianvirus(SV40)DNAReplicationAssayKitisthebestmodelformammalianchromosomalDNAreplicationinvitro(1)
AdditionofSV40LargeTAntigeninitiatesreplicationinthepresenceofhostproteins(2)

Applications

• examiningthefidelityandbiochemistryofDNAreplicationinhumans(3,4)
• identifyingmodeofactionandmechanismsleADIngtocelltransformationinvitroandtumorformationinvivo(5)
• regulatinggeneexpressionineukaryoticcells(2)
• studyingtheinhibitionofDNAreplicationbymutagenesis(6,7)
• determiningtherisksposedbyhumancarcinogens
• comparingreplicationofnormalandmalignantcells
• investigatingthecytotoxiceffectsofdrugs(8)
• investigatingapoptosis

Kitcontains:HeLacellextract,plasmidspUC-HSOandpUC-8-4,reactionbuffers,creatinephosphokinase,20xdNTPs/NTPs,phosphocreatine,yeastRNAcoprecipitant
SimplyaddSV40LargeTAntigenandyouhaveacompletesystemforinvitromodelingofchromosomalDNAreplication

Note

SV40LargeTAntigensoldseparately

QualityControl

AllpreparationsareassayedforstimulationofDNAsynthesisinaTantigen-dependentDNAreplicationassay(2)
Typicalpreparationsaregreaterthan90%pureasjudgedbySDSpolyacrylamidegelelectrophoresis

StorageConditions

Storeat-80°C
ShippedonDryIce

Downloads

MSDSPDF

References

1.Evan,G.andLittlewood,T.(1998)Science281,1317-1321
2.Wold,M.S.,Weinberg,D.H.,Virshup,D.M.,Li,J.J.andKelly,T.J.(1989)J.Biol.Chem.264,2801-2809
3.Thomas,D.C.,Veaute,X.,Kunkel,T.A.andFuchs,R.P.P.(1994)Proc.Natl.Acad.Sci.91,7752-7756
4.Boyer,J.C.,Thomas,D.C.,Maher,V.M.,McCormick,J.J.andKunkel,T.A.(1993)CancerRes.53,3270-3275
5.Bebenek,K.,Thomas,D.C.,Roberts,J.D.,Eckstein,F.andKunkel,T.A.(1993)Mol.Pharm.43,57-63
6.Stillman,B.(1994)Cell78,725-728
7.Fanning,E.(1992)J.Virol.66,1289-1293
8.Dixon,R.A.F.,andNathans,D.(1985)J.Virol.53,1001-1004