Details
Description
- MorepotentthancompetinghumanplacentalRNaseinhibitors
- RNase-free
- EffectiveoverbroadpHrangefrompH5.5to8.5
- Activeovertemperaturerangefrom37°Cto70°C
- RNaseInhibitordoesnotinterferewithSP6,T7,orT3RNApolymerase,AMVorM-MLVreversetranscriptaseorTaqDNApolymerase
- RNaseInhibitorincreasesthetimeRNAcanbesafelystored
UnitDefinition
Oneunitistheamountofribonucleaseinhibitorrequiredtoinhibittheactivityof5ngofRibonucleaseAat25°C(by50%)
RibonucleaseinhibitoractivityisdeterminedbytheinhibitionofRNaseAhydrolysisofcyclic2",3"-cytdinemonophosphateinaspectrophotometerrecordingassay
StorageBuffer
20mMHepes-KOH(pH7.5)
100mMKCl
0.1mMEDTA
50%(v/v)glycerol
AssayConditions
0.1MTris-acetate(pH6.5at22°C)
1mMEDTA
1mMcyclic2",3"-cytidinemonophosphate
Reactionvolumeof1ml
QualityControl
AllpreparationsaretestedforcontaminatingendonucleaseandexonucleaseandnonspecificRNaseandsingle-anddouble-strandedDNaseactivites
StorageContitions
Storeat-20°C
ProductshippedondryIce
Downloads
CertificateofAnalysisPDF-CurrentLot
MSDSPDF