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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Details
Source
Escherichiacoli
Applications
- MorethermalstablethanCalfIntestineAlkalinePhosphatase(CIP)
- Optimalincubationtemperatureisapproximately60°C,howevertheenzymeremainsactivefrom20°Cto80°C.
- Resistanttochemicalchangesandactiveoverabroadrangeofbufferconditions.
- Canbeusedtoremove5"-phosphatesfromDNAorRNApriorto5"-endlabeling(1).
- Workstoremove5"-phosphatesfromlinearizedvectormoleculestopreventself-ligationofthevectorduringcloningprocedures(1).
- Idealfordiagnosticimmunoassaysandimmunodetectionofproteinsandnucleicacidsfollowingblottingexperiments(1).
UnitDefinition
Oneunitistheamountofenzymerequiredtohydrolyze1molofp-nitrophenylphosphate37°Cforone(1)minuteinabufferconsistingof:
0.25mMMgCl2 (pH9.8).
1Mdiethanolamine.
10mMp-nitrophenylphosphate.
StorageBuffer
20mMTris-HCl(pH7.0at22°C)
5mMpotassiumphosphate
100mMKCl
0.1mMMgCl2
0.1mMZnCl2
+StABIlizers
QualityControl
AllpreparationsareassayedforcontaminatingendonucleaseandnonspecificRNaseandsingle-anddouble-strandedDNaseactivities
StorageConditions
Storeat-20°C
Productshippedondryice
References
(1)Sambrook,J.etal.(1989)Molecularcloning:AlaboratoryManual,secondedition,pp.1.56,5.72ColdSpringHarbor,NewYork
