Details
Source
Xanthomonasbadrii
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50µl
StarActivity
Yes
ReactionTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
Adenovirus2DNA
ReactionBuffer
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
1mMdithiothreitol
50mMNaCl
100µg/mlbovineserumalbumin
Note:blockedbyoverlappingDammethylation
ActivityinBuffers
Buffer1:25%
Buffer2: 100%
Buffer3:100%
Buffer4:75%
Buffer5: 50%
*Staractivity
StorageBuffer
50mMTris-HCl(pH7.4at4°C)
0.1mMEDTA
1mMdithiothreitol
500µg/mlBSA
50%(v/v)glycerol
AssayConditions
10mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1µgofAdenovirus2DNA
1mMdithiothreitol
100 µg/mlbovineserumalbumin
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF-CurrentLot
MSDSPDF-CurrentLot
Categories
RestrictionEndonucleases