Details
Source
ThermusThermophilus111
ReagentsSupplied
CMXBuffer1
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1 µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50 µl
StarActivity
N/A
ReactionTemperature
65°C
HeatInactivation
No
AssayUnitSubstrate
Adenovirus2DNA
ReactionBuffer
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
StorageBuffer
10mMTris-HCl(pH7.5at4°C)
200mMKCl
0.1mMEDTA
1mMdithiothreitol
200µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
1mMdithiothreitol
100 µg/mlbovineserumalbumin
1µgAdenovirus2DNA
Incubationisat65°Cfor1hourinatotalreactionvolumeof50 µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases