Details
Source
Thermus thermophilus 111
Reagents Supplied
CMX Buffer 1
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
N/A
Reaction Temperature
65°C
Heat Inactivation
No
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Storage Buffer
10 mM Tris-HCl (pH 7.5 at 4°C)
200 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
200 µg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg Adenovirus 2 DNA
Incubation is at 65°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
MSDS PDF - Current Lot
Categories
Restriction Endonucleases