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Chimerx/TspGW I/2501/250 u

价格
¥2760.00
货号:2501-01
浏览量:127
品牌:Chimerx
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商品描述
Details
Source

PurifiedfromEcolistrainthatcarriestheclonedtspGWRIgenefromThermusspeciesGW

UnitDefinition

Oneunitistheamountofenzymerequiredtodigest1 µgofpBR322DNAtoobtainastabledigestionpatternin1hourinatotalreactionvolumeof50 µl

ReactionTemperature

70°C

HeatInactivation

No

AssayUnitSubstrate

pBR322DNA

ReactionBuffer

10mMTris-HCl(pH8.5at25°C)
1mMdithiothreitol
10mMMgCl2 
+Enhancers(1) 

Notes:

  1. Avoidmultiplecyclesoffreezing/thawingofthestockreactionbuffer;Nomorethanthree[3]times.
  2. Thawingshouldbepreformedattemperaturesnotexceeding10°C.
  3. Recommendedprocedureistodividetheprovidedreactionbufferintosmallerportionsandpreservethemat-70°Cforlongtermstorage.
  4. Recommendedshorttermstorageofreactionbufferisat-20°C.
StorageBuffer

10mMTris-HCl(pH7.5at25°C)
0.1mMEDTA
50mMNaCl
1mMdithiothrietol
100µg/mlbovineserumalbumin
50%(v/v)glycerol

AssayConditions 

10mMTris-HCl(pH8.5at25°C)
10mMMgCl2 
1mMdithiothreitol
1µgpBR322DNA 
Incubationisat70°Cfor1hourinatotalreactionvolumeof50 µl

Notes
  1. Itisnotrecommendedtousemorethan2unitsper50 µlreaction.
  2. Digestionshouldbeperformedforovertwo[2]hours.
  3. AsTspGWIbindsDNAverytightly,excessamountofTspGWIaddedcanretardDNAmigrationonagel,eveninthepresenceofdenaturingagents.
  4. TspGWIisveryhighlystimulatedbythepresenceoftworestrictionsitesinoppositeorientation;Boththedistancebetweenrecognitionsequencesandtheirimmediateneighborhoodalsoaffectsthecleavageeffectiveness;Singlesitesubstratesarecleavedslowly.
StorageConditions

Storeat-20°C
Shippedondryice

References 

(1)Zylicz-Stachula,A.,Harasimowicz-Slowinska,R.,Sobolewski,I.andSkowron,P.M.(2002)NucleicAcidsResearch30,7e33

Downloads

CertificateofAnalysisPDF-CurrentLot

Categories

RestrictionEndonucleases

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