Details
TaqISource
Thermusaquaticus
ReagentsSupplied
CMXBuffer3
UnitDefinition
OneunitistheamountofenzymerequiredtocompletelydigestΦX-174DNAin1hourinatotalreactionvolumeof50μl
StarActivity
Yes
ReactionTemperature
65°C
HeatInactivation
No
AssayUnitSubstrate
ΦX-174DNA
ReactionBuffer
CMXBuffer3:
50mMTris-HCI(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:25%
Buffer2: 75%
Buffer3:100%
Buffer4:75%
Buffer5: 25%
*Staractivity
StorageBuffer
50mMTris-HCl(pH7.5at4°C)
300mMKCl
0.1mMEDTA
10mMβ-mercaptoethanol
0.1%TritonX-100™
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
50mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgΦX-174DNA
Incubationisat65°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF-CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases