Details
StuISource
Streptomycestubercidicus
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofumethylatedlamBDaDNAin1hourat37°Cinatotalreactionvolumeof50µl
StarActivity
No
ReactionTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 75%
Buffer2: 100%
Buffer3:50%
Buffer4:75%
Buffer5:75%
StorageBuffer
10mMTris‑HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
1mMdithiothreitol
0.5mg/mlbovineserumalbumin
0.1mMPMSF
50%(v/v)glycerol
AssayConditions
10mMTris-HCl(pH7.5at37°C)
50mMNaCl
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgunmethylatedlambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis PDF-CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases