Details
SphISource
StreptomycesphaeochromogenesReagentsSupplied
10XSphIReactionBuffer
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourinatotalreactionvolumeof50μlStarActivity
Yes
ReactionTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
10XSphIReactionBuffer:
50mMTris-HCl(pH7.4atRoomTemperature)
6mMMgCl2
50mMNaCl
50mMKCl
ActivityinBuffers
Buffer1: 25%
Buffer2: 50%
Buffer3:75%
Buffer4:25%
Buffer5:25%
*Staractivity
StorageBuffer
50mMTris‑HCl(pH7.5at4°C)
1mMEDTA
100mMKCl
5mMβ-mercaptoethanol
0.1%TritonX-100™
0.1mMPMSF
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
50mMTris‑HCl(pH7.4atRoomTemperature)
6mMMgCl2
50mMNaCl
50mMKCl
1µglambdaDNA
StorageConditions
Storeat-20°C
Shippedondryice
SphIDownloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases