Details
SpeISource
Sphaerotilusspecies
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
Ad-2DNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 100%
Buffer2: 100%
Buffer3:50%
Buffer4:75%
Buffer5:100%
*Staractivity
StorageBuffer
50mMTris-HCl(pH7.5at4°C)
200mMNaCl
0.1mMEDTA
0.2%(v/v)Triton
X-100™
1mMdithiothreitol
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1 µgAd-2DNA
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases