Details
PinAISource
Pseudomonasinaequalis
ReagentsSupplied
CMXBuffer5
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourintotalreactionvolumeof50µl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer5:
20mMTris-HCl(pH7.0at37°C)
5mMMgCl2
50mMKCl
1mMdithiothreitol
0.1mg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:50%
Buffer2: 75%
Buffer3:50%
Buffer4:50%
Buffer5:100%
StorageBuffer
20mMTris-HCl(pH7.5at4°C)
0.1mMEDTA
100mMNaCl
10mMβ-mercaptoethanol
0.05%(v/v)TritonX-100™
50%(v/v)glycerol
AssayConditions
20mMTris‑HCl(pH7.0at37°C)
5mMMgCl2
50mMKCl
1mMdithiothreitol
100 µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases