Details
NsiISource
Neisseriasicca
ReagentsSupplied
CMXBuffer3
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1 µgoflamBDaDNAin1hourat37°Cinatotalreactionvolumeof50 µl
StarActivity
No
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer3
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: <25%
Buffer2: 75%
Buffer3:100%
Buffer4:75%
Buffer5:50%
StorageBuffer
50mMTris-HCl(pH7.5at4°C)
200mMNaCl
0.1mMEDTA
1mMDTT
500µg/mlbovineserumalbumin
50%glycerol
AssayConditions
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases