Details
NruISource
Nocardiarubra
ReagentsSupplied
CMXBuffer3
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofunmethylatedlamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer3:
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
1mMdithiothreitol
100mMNaCl
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:0%
Buffer2: 25%
Buffer3:100%
Buffer4:25%
Buffer5:50%
StorageBuffer
50mMTris‑HCl(pH7.5at4°C)
100mMKCl
1.0mMEDTA
10mMβ-mercaptoethanol
500µg/mlbovineserumalbumin
0.1mMphenylmethylsulfonylfluoride
50%(v/v)glycerol
AssayConditions
50mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgunmethylatedlambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
NruIDownloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases