Details
NotISource
Nocardiaotitidis-caviarum
ReagentsSupplied
CMXBuffer3
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50 µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
Adenovirus2DNA
ReactionBuffer
CMXBuffer3:
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
100µg/mlbovineserumalbumin
1mMdithiothreitol
ActivityinBuffers
Buffer1:0%
Buffer2: 50%
Buffer3:100%
Buffer4:25%
Buffer5:25%
*Staractivity
StorageBuffer
20mMTris‑HCl(pH7.5at4°C)
100mMKCl
0.1mMEDTA
1mMdithiothreitol
0.01%(v/v)TritonX‑100™
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
50mMTris‑HCl(pH7.5at37°C)
100mMNaCl
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
1.0µgofAdenovirus2DNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
NotIDownloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases