Details
Nhe I Source
Neisseria mucosa heidelbergensis
Reagents Supplied
CMX Buffer 5
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
CMX Buffer 5:
20 mM Tris-HCl (pH 7.0 at 37°C)
5 mM MgCl2
50 mM KCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 100%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 75%
Buffer 5: 100%
*Star activity
Storage Buffer
10 mM Tris-HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
0.1 mM dithiothreitol
0.1% (v/v) Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
20 mM Tris-HCl (pH 7.0 at 37°C)
50 mM KCl
5 mM MgCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg Adenovirus 2 DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50µl
Quality Control
All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities
Notes:
- Due to star activity, it is not recommended to incubate over 4 hours or to use more than 100 units per reaction.
- Salt concentration above 50 mM inhibits Nhe I activity.
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases
