NheISource

Neisseriamucosaheidelbergensis

ReagentsSupplied

CMXBuffer5

UnitDefinition

Oneunitistheamountofenzymerequiredtocompletelydigest1 µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50 µl

StarActivity

Yes

IncubationTemperature

37°C

HeatInactivation

10minutesat65°C

AssayUnitSubstrate

Adenovirus2DNA

ReactionBuffer

CMXBuffer5:
20mMTris-HCl(pH7.0at37°C)
5mMMgCl₂
50mMKCl
1mMdithiothreitol
100µg/mlbovineserumalbumin

ActivityinBuffers

Buffer1: 100%
Buffer2: 100%
Buffer3:75%
Buffer4:75%
Buffer5:100%
*Staractivity 

StorageBuffer

10mMTris-HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
0.1mMdithiothreitol
0.1%(v/v)TritonX-100™
500µg/mlbovineserumalbumin
50%(v/v)glycerol

AssayConditions 

20mMTris-HCl(pH7.0at37°C)
50mMKCl
5mMMgCl₂ 
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgAdenovirus2DNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl

QualityControl

Allpreparationsareassayedforcontaminatingendonuclease,3"-exonuclease,andnonspecificsingle-anddouble-strandedDNaseactivities

Notes:

1. Duetostaractivity,itisnotrecommendedtoincubateover4hoursortousemorethan100unitsperreaction.
2. Saltconcentrationabove50mMinhibitsNheIactivity.

StorageConditions

Storeat-20°C
Shippedondryice 

Downloads

CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot

Categories

RestrictionEndonucleases