Nde I Source

Neisseria denitrificans

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin 

Activity in Buffers

Buffer 1: 50%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 100%
Buffer 5: 75%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
0.1 mM EDTA
1 mM DTT
50 mM KCl
500 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

20 mM Tris‑acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA 

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases