Details
Source
Micrococcusluteus
ReagentsSupplied
CMXBuffer3
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer3:
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 25%
Buffer2:75%
Buffer3:100%
Buffer4:50%
Buffer5:50%
*Staractivity
StorageBuffer
50mMTris‑HCl(pH7.4at4°C)
50mMNaCl
0.1mMEDTA
1mMdithiothreitol
500µg/mlbovineserumalbumin
0.1%TritonX-100™
50%(v/v)glycerol
AssayConditions
50mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100 µg/mlbovineserumalbumin
1μglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnaysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases