Details
Source
Haemophilusparainfluenzae
ReagentsSupplied
CMXBuffer4
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourat37°Cinatotalreactionvolumeof50µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
No
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer4:
20mMTris-Acetate(pH7.5at37°C)
50mMpotassiumacetate
10mMmagnesiumacetate
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:25%
Buffer2:50%
Buffer3:25%
Buffer4: 100%
Buffer5:100%
*Staractivity
StorageBuffer
20mMTris-HCl(pH7.4at4°C)
0.5mMEDTA
50mMKCl
1mMDTT
0.1%(w/v)TritonX-100™
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
20mMTris‑Acetate(pH7.5at37°C)
50mMpotassiumacetate
10mMmagnesiumacetate
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot