Details
Source
HaemophilusinfluenzaeRf
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourat37°Cinatotalreactionvolumeof50µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
80°Cfor10minutes
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:25%
Buffer2:100%
Buffer3:75%
Buffer4:75%
Buffer5:50%
*Staractivated
StorageBuffer
50mMTris-HCl(pH7.5at4°C)
0.1mMEDTA
10mMβ-mercaptoethanol
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
10mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
Reactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot