Details
Source
HaemophilusinfluenzaeRc
ReagentsSupplied
CMXBuffer5
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
65°Cfor10minutes
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer5:
20mMTris-HCl(pH7.0at37°C)
5mMMgCl2
50mMKCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:50%
Buffer2:50%
Buffer3:75%
Buffer4:>75
Buffer5:100%
StorageBuffer
10mMTris‑HCl(pH7.4at4°C)
200mMNaCl
0.1mMEDTA
1mMdithiothreitol
500μg/mlbovineserumalbumin
5μg/mlpoly-L-lysine
0.1%TritonX-100™
50%(v/v)glycerol
AssayConditions
1mMDTT
100μg/mlBSA
20mMTris‑HCl(pH7.0at37°C)
50mMKCl
5mMMgCl2
1μglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50μl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot