Details
Source
Haemophilushaemolyticus
ReagentsSupplied
CMXBuffer3
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1 μgoflamBDaDNAin1hourat37°Cinatotalreactionvolumeof50 µl
StarActivity
No
HeatInactivation
65°Cfor10minutes
IncubationTemperature
37°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer3:
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100μg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:25%
Buffer2:100%
Buffer3:100%
Buffer4:75%
Buffer5:50%
StorageBuffer
20mMTris-HCl(pH7.5at4°C)
100mMKCl
0.5mMDTT
500µg/mlbovineserumalbumin
0.1%(w/v)TritonX-100™
50%glycerol
AssayConditions
50mMTris-HCl(pH7.5at37°C)
10mMMgCl2
100mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
Reactionvolumeis50µl
Incubationisat37°C for1hour
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot