Details
Source
Haemophilusaegyptius
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
80°Cfor10minutes
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer2: 100%
*Staractivity
StorageBuffer
50mMTris‑HCl(pH7.5at4°C)
100mMKCl
0.5mMdithiothreitol
500µg/mlbovineserumalbumin
50%(w/v)glycerol
AssayConditions
10mMTris-HCl(pH7.5at37°C)
10mM MgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis PDF -CurrentLot
MSDSPDF -CurrentLot