Details
Source
Deinococcus radiophilus
Reagents Supplied
CMX Buffer 4
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
65°C for 10 minutes
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 100%
Buffer 5: 0%
Storage Buffer
50 mM Tris‑HCl (pH 7.4 at 4°C)
50 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
500 µg/ml bovine serum albumin
0.1 mM phenylmethylsulfonyl fluoride
50% (v/v) glycerol
Assay Conditions
See Certificate of Analysis Below
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
Certificate of Analysis
Lot 2709013 PDF
MSDS
MSDS PDF