Source

Purified from an E coli strain that carries the DpnI gene from Dipplococcus pneumoniae 

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of pBR322 dam methylated DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

No

Inactivation Temperature

80°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

pBR322 dam methylated DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 100%
Buffer 5: 0%

Storage Buffer 

10 mM Tris-HCl (pH 7.4 at 4°C)
400 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
0.1% Trition X-100™
200 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Notes

1. Dpn I cleaves only methylated GATC sites.
2. It may be necessary to add more enzyme to obtain complete digestion when using other buffer than optimal (Acet).
3. It is recommended to add 10 units of the enzyme to obtain complete digestion of the methylated template after standard site-directed mutagenesis (total reaction volume is 50 μl).

Storage Conditions

Store at -20°C
Shipped on dry ice

Downloads

Certificate of Analysis
Lot 2407019 PDF

MSDS
MSDS PDF