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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
Source
PurifiedfromanEcolistrainthatcarriestheDpnIgenefromDipplococcuspneumoniae
ReagentsSupplied
CMXBuffer4
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1 μgofpBR322dammethylatedDNAin1hourinatotalreactionvolumeof50 μl
StarActivity
No
InactivationTemperature
80°Cfor10minutes
IncubationTemperature
37°C
AssayUnitSubstrate
pBR322dammethylatedDNA
ReactionBuffer
CMXBuffer4:
20mMTris-acetate(pH7.5at37°C)
10mMmagnesiumacetate
50mMpotassiumacetate
1mMdithiothreitol
ActivityinBuffers
Buffer1:75%
Buffer2:100%
Buffer3:100%
Buffer4: 100%
Buffer5:0%
StorageBuffer
10mMTris-HCl(pH7.4at4°C)
400mMNaCl
0.1mMEDTA
1mMdithiothreitol
0.1%TritionX-100™
200μg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
SeeCertificateofAnalysisBelow.
Notes
- DpnIcleavesonlymethylatedGATCsites.
- Itmaybenecessarytoaddmoreenzymetoobtaincompletedigestionwhenusingotherbufferthanoptimal(Acet).
- Itisrecommendedtoadd10unitsoftheenzymetoobtaincompletedigestionofthemethylatedtemplateafterstandardsite-directedmutagenesis(totalreactionvolumeis50μl).
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis
Lot2407019PDF
MSDS
MSDSPDF
