Details
Source
ChlorellavirusIL-3A
Note1:PurifiedfromEcolistrainthatcarriestheclonedcvijRIgenefromChlorellavirusIL-3A(PatentNo.US005472872A)
ReagentsSupplied
10XCviJIReactionBuffer
DMSO
Applications
- CviJIistheonlyavailablerestrictionendonucleaserecognizingtwo-threebasepairsequence(1,2,3),thusallowingfornewresearchapplications(4)
- ThisrecombinantversionofCviJIcleavesonlyPuGCPysites(4)
- SuitableforhighresolutionmappingofshortDNA"slikeamplifiedproductsorsmallplasmids
- OtherCviJIapplications,likeshotguncloning,thermalcyclelabeling(5)orepitopemappingcanbeperformedeitherwiththisversionorwithCviJI*
- ExclusivelyfromChimerx
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofpBR322DNAin1hourinatotalreactionvolumeof25µl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
65°Cfor 10minutes
AssayUnitSubstrate
pBR322
ReactionBuffer
10XCviJIReactionBuffer:
20mMglycylglycine-KOH(pH8.5)
10mMmagnesiumacetate
50mMpotassiumacetate
0.1mMdithiothreitol
Note2:100%DMSOsuppliedseparately
AssayConditions
20mMglycyl-glycine-KOH(pH8.5)
50mMpotassiumacetate
10mMmagnesiumacetate
0.1mMdithiothreitol
10%DMSO
1µgpBR322
Incubationfor1hourat37°Cinareactionvolumeof25µl
StorageBuffer
20mMTris-acetate(pH8.0at4°C)
50mMpotassiumacetate
5mMMgCl2
0.5mMEDTA
1mMdithiothreitol
50%(v/v)glycerol
StorageConditions
Storeat-80°C
Shippedondryice
Downloads
CerftificateofAnalysisPDF-CurrentLot
References
(1)Xia,Y.,Burbank,D.,Uher,L.,Rabussay,D.andVanEtten,J.NucleicAcidsRes.15,6075-6090
(2)Fitzgerald,M.C.,Skowron,P.,VanEtten,J.L.,Smith,L.M.andMead,D.A.(1992)NucleicAcidsRes.20,3753-3762
(3)Mead,D.,Swaminathan,N.,VanEttenJ.andSkowron,P.M.:RecombinantCviJIrestrictionendonuclease.(1995)UnitesStatesPatentnoUS005472872A
(4)Skowron,P.M,Swaminathan,N.,McMaster,K.,George,D.,VanEtten,J.andMead,D.Gene157(1995)37-41
(5)Swaminathan,N.,McMaster,K.,Skowron,P.andMead,D.A.AnalyticalBiochemistry255(1998)133-141
