Details
Source
CaryophanonlatumL
ReagentsSupplied
CMXBuffer4
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
No
IncubationTemperature
37°C
AssayUnitSubstrate
LambdaDNA
HeatInactivation
65°Cfor10minutes
Note
ClaIisblockedbydammethylation
ReactionBuffer
CMXBuffer4:
20mMTris-acetate (pH7.5at37°C)
50mMpotassiumacetate
10mMmagnesiumacetate
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:75%
Buffer2:100%
Buffer3:100%
Buffer4: 100%
Buffer5:100%
StorageBuffer
50mMTris-HCl(pH7.4at4°C)
0.1mMEDTA
200mMKCl
5mM β-mercaptoethanol
0.1%(v/v)TritonX-100™
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
SeeCertificateofAnalysisBelow
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis
Lot2102024PDF
MSDS
MSDSPDF