Details
Source
BacillusstearoThermophilusHKC
Note:PurifiedfromEcolistrainthatcarriestheclonedbsiHKCRIgene.
ReagentsSupplied
CMXBuffer3
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1μgoflamBDaDNAin1hourinatotalreactionvolumeof50μl
StarActivity
Yes
IncubationTemperature
55°C
HeatInactivation
No
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer3:
50mMTris-HCl(pH7.5at37°C)
100mMNaCl
10mMMgCl2
1mMDTT
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:<25%
Buffer2:75%
Buffer3:100%
Buffer4:75%
Buffer5:50%
*staractivity
StorageBuffer
10mMTris-HCl(pH7.5at4°C)
50mMKCl
1mMdithiothreitol
0.1mMEDTA
500 µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
SeeCertificateofAnalysisBelow
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis
Lot2206007PDF
MSDS
MSDSPDF