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Chimerx/Tth DNA Polymerase/1115/5,000 u

价格
¥32000.00
货号:1115-02
浏览量:127
品牌:Chimerx
服务
全国联保
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商品描述
Details
ReagentsSupplied

10XTthDNAPolymeraseReactionBuffer

Source

ThermusThermophilus

Applications
  • Ccanreversetranscribeatelevatedtemperatures
  • MinimizesproblemswithstrongsecondarystructureofRNA
  • Resultsingreaterspecificityofprimerhybridizationandextension
  • SuitableforhightemperaturesynthesisofDNA
  • SynthesizesCDNAfromRNAtemplate
  • StablethermophilicDNApolymerase(1)
UnitDefinition

Oneunitistheamountofenzymerequiredtoincorporate10nmolestotalnucleotideintoacidinsolublematerialat70°Cfor30minutes.

Concentration

5-10units/µl

StorageBuffer

50mMTrisHCl(pH7.5) 
0.1mMEDTA
5.0mMdithiothreitol
50%(v/v)glycerol 
StABIlizers

AssayConditions

25mMTris-HCl(pH9.5at25°C)
50mMpotassiumchloride
10mMmagnesiumchloride
1mMdithiothreitol
0.3µg/μlactivatedcalfthymusDNA
1μg/μl bovineserumalbumin
0.2mMofeachdCTP,dGTP,dTTPand[α-32P]dATP
Incubationisfor10minutesat70°C inatotalreactionvolumeof50µl

StorageConditions

Storeat-20°C
Productshippedondryice

References

(1)Wang,A.M.,Doyle,M.V.,andMark,D.F.(1989)Proc.Nat’l.Acad.Sci.USA86,9717-9721

Downloads

CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot  

Categories

Polymerases,MesophilicDNAPolymerases

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