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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
Source
Thermusflavus
Applications
- EfficientlysynthesizesDNAatelevatedtemperatures(1)
- CanbeusedforamplificationofnucleicacidsinPCRandLCRreaction
- UsedforhightemperatureDNAsequencing
- BroaderMg2+ionsconcentrationtolerancethanTaqDNApolymerase
ReagentsSupplied
10XTflDNAPolymeraseReactionBuffer
UnitDefinition
Oneunitistheamountofenzymerequiredtoincorporate10nmolestotalnucleotideintoacidinsolublematerialat70°Cfor30minutes
Concentration
3.0units/µl
StorageBuffer
50mMTrisHCl(pH7.5)
0.1mMEDTA
5mMdithiothreitol
50%(v/v)glycerol
StABIlizers
AssayConditions
25mMTris-HCl(pH9.5at25°C)
50mMpotassiumchloride
10mMMgCl2
1mMdithiothreitol
1mg/mlbovineserumalbumin
300µg/mlactivatedcalfthymusDNA
0.2mMofeachdCTP,dGTP,dTTPand[α-32P]dATP
Incubationisfor10minutesat70°C inareactionvolumeof50µl
StorageConditions
Storeat-20°C
Productshippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF
References
1.Kaledin,A.S.,Sliusarenko,A.G.andGorodetskii,S.I.(1980)Biokhimiya45,644-651
