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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
Details
Source
Thermusaquatieus
Applicatons
- Hydrolyzespyrophosphate,acommonproductofbiosyntheticreacions(1)
- Maintainstheforwarddirectionofreactionsgeneratingpyrophosphate
- ImprovesPCRamplificationofproblematicoflongtemplates
- UsedasanenzymelabelofELISA(2)
UnitDefinition
Oneunitwillliberate1.0nmolofinorganicorthophosphateperminuteat65°C(pH7.5)
StorageBuffer
20mMTris-HCl(pH8.0at4°C)
50mMimidazole
100mMKCl
0.1mMEDTA
50%(v/v)glycerol
0.5%TritonX-100
AssayConditions
40mMTris-HCl(pH7.5at22°C)
40mMsodiumacetate
40mMimidazole
1.0mMsodiumpyrophosphate.
2.5mMMgCl2
Reactionvolume100 μl
QualityControl
Allpreparationsareassayedforcontaminatingendonuclease,exonucleaseandnonspecificRNaseandsingle-anddouble-strandedDNaseactivites.
StorageConditions
Storeat-80°C
ShippedonDryIce
Downloads
MSDSPDF -CurrentLot
References
Baykov,A.A.,Kasho,V.N.andAvaeva,S.M.(1988)Anal.Biochem.171,271-276.2.Jerhoeven,J.A.(1986)J.Bacteriol.168,318-321
Richter,O.M.andSchafer,G.(1992)Eur.J.Biochem.209,343-349
