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| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
□ HRP-Juice, two bottle
Our stabilized ultra-sensitive Luminol based substrates detect horseradish peroxidase at femtogram level and our chemiluminescent systems 3 to 5 more times sensitive than our best competitor. These reagents are stable for 21 days after mixing when stored at 4°C.
□ Additional Informations
Storage : Store the reagent bottles,HRP-A and HRP-B, at 4-8 °C.Usage : Mix equal volumes of HRP-A and HRP-B in a clean container andequilibrate at room temperature for 30 minutesbefore use. Do not contaminatethe HRP enzyme substrate with HRP enzyme or other proteins.HRP enzyme substratehas a wide range to detect HRP enzyme in solution as well as in ELISA. Thelower enzyme concentration may be a few femto grams and the highestconcentration HRP of enzyme may be one nano gram depending on the source of theHRP enzyme.For good results, wash your tube or micro titer plate or membrane with 0.2Mphosphate buffer, pH 8.4 to 8.6, and then use the substrate. Best results forchemiluminescence can be obtained from 2 minutes to 20 minutes after contactingsubstrate with HRP enzyme.
□ Alkaline Phosphatase
AP Juice
PJK´s series of stabilizedchemiluminescent 1, 2-dioxetanes substrates can detect AP enzyme at theattogram level over a period of a few minutes.
● Blotting (Nitrocellulose Membrane, PDVF, Nylon)
● Reporter Gene Assays
● Immunoassays
● Quantitative PCR
Advantages of Chemiluminescent Substrates for AP:
● Formulated for two wavelength (450nm and 540nm) Safe- contains no organic solvents and no radioactive
materials.
● Unsurpassed signal-up to 15 times brighter than leading commercial reagents.
● Low background-high signal to noise ratio allows increased signal without sacrifice.
●Steady glow-long lasting light emission without signal decay for up to four hours..
● Convenient-ready to use single bottle reagent requires no additional enhancers.
● Consistent results-reproducible results and ideal for automatic or manual systems.
● Very sensitive-less than one attogram of alkaline phosphatase has been detected.
●Wide dynamic range-5 to 6 log dynamic range standard curves over a broad range of times.
● Detection platforms-solution phase applications, membrane based applications and single tube or microplate
format applications.
Alkaline phosphatase (orthophosphoric monoester phosphohydrolase, alkalineoptimum, AP) are found primarily in animal tissue and microorganism. AP used inenzyme immunoassays (EIA) are isolated from bovine intestinal mucosa or from E.coli. These enzymes have considerable differences in thier properties andshould not be assayed under identical conditions. The bacterial enzyme haslower activity than the bovine intestinal enzyme.Alkaline phosphatases hydrolyses numerous esters, such as those of primary andsecondary alcohols, phenols and amines.A major reason for the popularityof alkaline phosphatases for EIA is ist absence from higher plants. The enzymeis abundant in animals and human tissues involved in nutrient transport and indeveloping tissues and secretor organs, but it is not found in significantamounts in muscle, connective tissue or cartilage. Some pathological conditionsincrease alkaline phosphatase activities in sera.The enzyme transfers the phosphoryl residue via phosphoryl-enzyme intermediate,which can be repressed by organic phosphate. The comparative dtection limit ofAP using fluorescence, time-resolved fluorescence and colorimetric techniquesare 10 - 19M (6x104 molecules), 3 x 10-19M (1.8 x 105molecules) and 5 x 10-17M (3 x 108 molecules), respectively.Stabilized 1, 2-dioxetane substrates provide high signal, low background, widedynamic range, rapid results and exellent reproducibility. These 1, 2-dioxetanesprovide substrates which are highly sensitive but can detect an enzymeconcentration up to 10-21M (6x102 molecules of AP) in solution aswell as on membrane.
□ Components
HRP-Juice | |
| 1. HRP-A | 50ml |
| 2. HRP-B | 50ml |
AP-Juice with Enhancer,450nm LB | |
| 1. AP-Juice Enh. 450 nm LB | 100 ml |
: Alkaline Phosphatase Substrate with Enhancer-450nm in bufferwith Low Background | |
AP-Juice withoutEnhancer, 1x Low Background | |
| 1. AP-Juice without Enhancer, 1 x LB | 100 ml |
| : Alkaline Phosphatase Substrate in buffer without Enhancer. | |
