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abfrontier/Guide-it Cas9 Poly/Mono clonal Antibody/Guide-it Cas9 Polyclonal Antibody, 3 x 100 ul/632628

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货号:632628
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品牌:abfrontier
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주문정보

견적신청주문하기장바구니관심상품
  • - 재고수량은 변동될 수 있습니다.
  • - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
  • - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
선택Cat.No.제품명가격(VAT별도)수량
632628Guide-it Cas9 Monoclonal Antibody (Clone TG8C1), 100 ug로그인후 확인가능 합니다.
632627Guide-it Cas9 Monoclonal Antibody (Clone TG8C1), 3 x 100 ug로그인후 확인가능 합니다.
632607Guide-it Cas9 Polyclonal Antibody, 100 ul로그인후 확인가능 합니다.
632606Guide-it Cas9 Polyclonal Antibody, 3 x 100 ul로그인후 확인가능 합니다.

제품특징

□ 특징

● Cas9 nuclease 검출을 위한 rabbit polyclonal antibody & mouse monoclonal antibody

● Polyclonal Antibody는 Wild-type의 Cas9 뿐만 아니라 변형 Cas9 (Variants)도 인식 가능

● 높은 특이성: mammalian cell lysate에 의한 background가 현저히 낮음

● 고감도: Western blot으로 불과 0.5 ~ 1ng 의 재조합 Cas9 nuclease를 검출할 수 있으며 Cas9 발현 세포 3,500개까지의 signal 인식 가능

□ 제품설명

Guide-it™ Cas9Polyclonal Antibody본 제품은 Streptococcuspyogenes유래의 Cas9 단백질 검출을 할 수 있는 rabbit polyclonal antibody이며, 이는 Cas9 variants를 인식 가능하다 (Wild-type Cas9, Cas9 nickase, Cas9 nuclease-deficient mutants). 본 항체를 이용하면 Western blot 방법으로 대부분의 mammalian cell line에서 고감도, 낮은 background로 최소 0.5 ng의 재조합 Cas9을 검출 가능하다. Guide-it™ Cas9 Monoclonal Antibody (Clone TG8C1) 본 제품은 Streptococcus pyogenes 유래의 full-length Cas9 재조합 단백질을 검출할 수 있는 mouseantibody이며 hybridoma cells로부터 생성되었다. 본 항체는 western blot으로 mammalian cell lysate 또는 bacterial celllysate로부터 최소 1ng의 야생형(wilde-type)의 Cas9 단백질을 인식할 수 있다.그림1. The sensitivity of Cas9 polyclonal antibody assessed by Western blot. Various amounts ofrecombinant Cas9 (rCas9) were loaded (Lane 3:0.15 ng, Lane 4: 0.30 ng, Lane 5: 0.625 ng, Lane 6: 1.25 ng, Lane 7: 2.5 ng,Lane 8: 5.0 ng, Lane 9: 10.0 ng, Lane 10: 20.0 ng). Cell lysates prepared from3.5 x 104 cultured cells (Lane 1: HT1080 cells, Lane 2: HEK 293cells) were used as a negative control. The blot was probed with Cas9polyclonal antibody (1:1,000 dilution). For detection, an HRP-conjugated goatanti-rabbit secondary antibody was used (1:5,000 dilution), and signal wasdetected using ECL reagent.그림2. The specificity of Cas9 polyclonal antibody assessed by Western blot. Lysates prepared from various untransfected cell lines (3.5 x 104cultured cells; Lane 1: HepG2, Lane 2: CHO-K1, Lane 3: HeLa, Lane 4: MCF7,Lane5: HEK 293, Lane 6: HT1080, Lane 7: NIH 3T3, Lane 8: no cells) and HEK 293cells expressing Cas9 from the CMV promoter (stable cell line; Lane 10: 2.0 x103 cells, Lane 11: 5.0 x 103 cells, Lane 12: 1.0 x 104 cells,Lane 13: 1.5 x 104 cells). One nanogram of recombinant Cas9(rCas) was used as a positive control (Lane 9). The blot was probed with Cas9polyclonal antibody (1:1,000 dilution). For detection, an HRP-conjugated goatanti-rabbit secondary antibody was used (1:5,000 dilution), and signal wasdetected using ECL reagent. 그림 3. Western blot analysisof the Guide-it™ Cas9 Monoclonal Antibody (Clone TG8C1). The Guide-it Cas9Monoclonal Antibody (Clone TG8C1), which was raised in mouse againstrecombinant full-length Cas9 (rCas9), was tested via Western blot analysisusing either increasing concentrations of recombinant Cas9 (Panel A) or lysatesmade from cells transiently expressing Cas9 after plasmid transfection (PanelB). Panel A: Increasing concentrations of rCas9 (1 ng, 2 ng, 3 ng, and 5 ng)were analyzed via Western blotting. Panel B: HEK 293 cells were transientlytransfected with a plasmid encoding Cas9 protein.

□ 적용

Western blot detection of Cas9 proteinImmunocytochemistry detection of Cas9 protein

□ 보존

-20 ℃

□ 구성품

Code

구성품

632607

100 μl Guide-it Cas9 PolyclonalAntibody (1 μg/μl)

632606

3 x 100 μl Guide-it Cas9Polyclonal Antibody (1 μg/μl)

632628

100 μg Guide-it Cas9 Monoclonal Antibody(Clone TG8C1) (1 μg/μl)

632627

3 x 100 μg Guide-it Cas9 MonoclonalAntibody (Clone TG8C1) (1 μg/μl)

페이지 맨 위로 가기
abfrontier的TBGreen®快速qPCR混合液是使用TBGreen®插入实时PCR 的  试剂。该产品通过结合适用于高速反应的修饰Taq DNA聚合酶和针对抗Taq抗体优化的反应组合物,实现了低引物二聚体的高速反应。另外,可以再现地进行大范围目标的更准确的定量和检测。将Tli RNaseH(耐热RNaseH)添加到2x Premix试剂中,因此,如果以cDNA为模板,则可将残留mRNA对PCR反应的抑制作用降至最低。通过改善酶和反应缓冲液的组成,可以扩增长靶标和具有高GC含量的靶标,并对PCR抑制剂表现出较强的抵抗力。它是2倍浓度的预混合型试剂,并且预先包含适合实时监控的适当浓度的  TBGreen®,因此反应溶液的制备非常简单。