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蚂蚁淘在线 / 品牌中心 / Abeomics / Abeomics/PD-1 Stable Cell Line/For Profit/14-500ACL

Abeomics/PD-1 Stable Cell Line/For Profit/14-500ACL

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货号：14-500ACL
浏览量：127
品牌：Abeomics
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Amount :Non Profit

PD-1 Stable Cell Line is a stably transfected CHO-K1 cell line which expresses human Programmed Cell Death Protein -1 (PD-1, also known as CD279).

Sequence data: hPD-1 (accession numberNP_005009)

MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTS
ESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGT
YLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGS
LVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVP
CVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL
Content :Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO
Storage condition :Immediately upon receipt, store in liquid nitrogen.

Application:.

  • Screen for antibodies of human PD-1 through Flow Cytometry.

Culture conditions:

Cells should be grown at 37oC with 5%CO2using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS supplemented with 10% FBS and 1% Pen/Strep, plus 10µg/ml of Blasticidin. (Note: Do not add Blasticidin to the parental CHO-K1 (Part #14500CCL) cell culture!)
It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37oCwater-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth medium without Blasticidin, transfer resuspended cells to T25 flask and culture in37oC-CO2incubator.
Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation.At first passage, switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence.
To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.To achieve satisfactory results, cells should not be passaged over 16 times.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Abeomics的人PD-L1重组Fc融合蛋白（正在供货）CHO-K1 Celline。 PD-L1（CD274 / B7-H1）是一种重要的膜结合共刺激分子，属于B7超家族，可通过其受体抑制免疫反应，PD-1和PD-L1在炎症性疾病的发病机理中起关键作用死亡1）。它在单核吞噬细胞系统（MPS）中广泛表达，可共同刺激T细胞并调节炎症反应。PD-L1通过对T细胞功能产生负面共刺激作用来发挥炎症调节功能，以抑制细胞因子分泌，促进活化T细胞凋亡并诱导T细胞无反应。在细菌感染，炎症和许多自身免疫性疾病中，已经报道了CD274的异常表达和失调。