快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Abeomics / Abeomics/TNF-beta Leeporter™ Luciferase Reporter-RAW264.7 Cell Line/For Profit/14-108ACL

Abeomics/TNF-beta Leeporter™ Luciferase Reporter-RAW264.7 Cell Line/For Profit/14-108ACL

价格
面议
货号：14-108ACL
浏览量：127
品牌：Abeomics
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • Description
  • Product Info
  • Application Note
  • Review   (0)
Amount :Non Profit

The TNF-beta Leeporter™ Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-beta promoter. Tumor necrosis factor-beta (TNF-beta) one of the major proinflammatory cytokines, which is produced predominantly by mitogen-stimulated T lymphocytes and leukocytes. TNF-beta is involved in the regulation of various biological processes such as cell proliferation, differentiation and apoptosis. TNF-beta gene polymorphisms are also significantly associated with disease susceptibility and phenotype in patients with psoriatic arthritis. The TNF-beta induction by lipopolysaccharide (LPS), the Toll-like receptor 4 (TLR4) ligand, is shown in Figure 1.

Content :Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :Immediately upon receipt, store in liquid nitrogen.

Application:

  • Monitor the TNF-beta induction activity.
  • Screen for activators or inhibitors of the TNF-beta signaling pathway.

Culture conditions:

Cells should be grown at 37oC with 5%CO2using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3µg/ml of Puromycin(Note: Puromycin can be omitted during the reporter cell assays).
It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37oCwater-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in37oC-CO2incubator.
Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation.At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.Note: RAW264.7 cells may not be detached well by trypsinization only. So you may need to use a cell scraper to harvest the trypsinized cells.
To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.To achieve satisfactory results, cells should not be passaged over 16 times.
Functional validation:

A. Response of TNF-beta Leeporter™ – RAW264.7 cells to lipopolysaccharide (LPS).

1. Plate TNF-beta Leeporter™ – RAW264.7 cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 1 x 105 cells/well and incubate cells at 37oCin a CO2 incubator for 4-6 hours.
2. Stimulate cells with different concentrations of LPS and incubate cells at 37oC in a CO2 incubator for 16 hours. ,
3. Equilibrate the plate to room temperature for 10 minutes.
4. Add 50 µl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well.
5. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

There are currently no product reviews
Write a review on this product!

Customers who purchased this product also purchased
TNF-beta Leeporter™ Luciferase Reporter-HEK293 Cell Line

TNF-beta Leeporter™ Lucifera...

details-TNF-beta Leeporter™ Luciferase Reporter-HEK293 Cell Line
TNF-alpha Leeporter™ Luciferase Reporter-RAW264.7 Cell Line

TNF-alpha Leeporter™ Lucifer...

details-TNF-alpha Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
TNF-alpha Leeporter™ Luciferase Reporter-HEK293 Cell Line

TNF-alpha Leeporter™ Lucifer...

details-TNF-alpha Leeporter™ Luciferase Reporter-HEK293 Cell Line
MIP-2 Leeporter™ Luciferase Reporter-RAW264.7 Cell Line

MIP-2 Leeporter™ Luciferase ...

details-MIP-2 Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
MIP-2 Leeporter™ Luciferase Reporter-HEK293 Cell Line

MIP-2 Leeporter™ Luciferase ...

details-MIP-2 Leeporter™ Luciferase Reporter-HEK293 Cell Line
AP-1 Leeporter™ Luciferase Reporter-HEK293 Cell Line

AP-1 Leeporter™ Luciferase R...

details-AP-1 Leeporter™ Luciferase Reporter-HEK293 Cell Line
IL-8 Leeporter™ Luciferase Reporter-RAW264.7 Cell Line

IL-8 Leeporter™ Luciferase R...

details-IL-8 Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
NF-kB Leeporter™ Luciferase Reporter-RAW264.7 Cell Line

NF-kB Leeporter™ Luciferase ...

details-NF-kB Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
Human Peripheral Blood CD19/CD27 Memory B Cells

Human Peripheral Blood CD19/CD...

details-Human Peripheral Blood CD19/CD27 Memory B Cells
Human Peripheral Blood CD19 B Cells

Human Peripheral Blood CD19 B ...

details-Human Peripheral Blood CD19 B Cells
Human Peripheral Blood CD14 Monocytes

Human Peripheral Blood CD14 Mo...

details-Human Peripheral Blood CD14 Monocytes
Human Peripheral Blood CD8 Cytotoxic T Cells

Human Peripheral Blood CD8 Cyt...

details-Human Peripheral Blood CD8 Cytotoxic T Cells
Human Peripheral Blood CD8/CD45RA Naive Cytotoxic T Cells

Human Peripheral Blood CD8/CD4...

details-Human Peripheral Blood CD8/CD45RA Naive Cytotoxic T Cells
Human Peripheral Blood CD4 Helper T Cells

Human Peripheral Blood CD4 Hel...

details-Human Peripheral Blood CD4 Helper T Cells

Most viewed Products

Recombinant Human Thioredoxin-Like 4A

Recombinant Human Thioredoxin-Like ...

details-Recombinant Human Thioredoxin-Like 4A
Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)

Monoclonal antibody to Human PD-L1 ...

details-Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)
Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)

Monoclonal Antibody to Caspase-3 (P...

details-Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)
Polyclonal Antibody to Beta actin

Polyclonal Antibody to Beta actin

details-Polyclonal Antibody to Beta actin
Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Monoclonal Antibody to GAPDH (Clone...

details-Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to Human IL-1beta (Clone: ABM26G5)

Monoclonal Antibody to Human IL-1be...

details-Monoclonal Antibody to Human IL-1beta (Clone: ABM26G5)
Monoclonal antibody to B7-H4 (Clone: ABM53A6)

Monoclonal antibody to B7-H4 (Clone...

details-Monoclonal antibody to B7-H4 (Clone: ABM53A6)
Polyclonal Antibody to Beta Tubulin

Polyclonal Antibody to Beta Tubulin

details-Polyclonal Antibody to Beta Tubulin
Peroxidase conjugated Goat anti Mouse IgG (H+L)

Peroxidase conjugated Goat anti Mou...

details-Peroxidase conjugated Goat anti Mouse IgG (H+L)
Monoclonal Antibody to EpCAM (CD326) Cytoplasmic Domain (Clone: ABM2C92)

Monoclonal Antibody to EpCAM (CD326...

details-Monoclonal Antibody to EpCAM (CD326) Cytoplasmic Domain (Clone: ABM2C92)
Abeomics的人PD-L1重组Fc融合蛋白（正在供货）CHO-K1 Celline。 PD-L1（CD274 / B7-H1）是一种重要的膜结合共刺激分子，属于B7超家族，可通过其受体抑制免疫反应，PD-1和PD-L1在炎症性疾病的发病机理中起关键作用死亡1）。它在单核吞噬细胞系统（MPS）中广泛表达，可共同刺激T细胞并调节炎症反应。PD-L1通过对T细胞功能产生负面共刺激作用来发挥炎症调节功能，以抑制细胞因子分泌，促进活化T细胞凋亡并诱导T细胞无反应。在细菌感染，炎症和许多自身免疫性疾病中，已经报道了CD274的异常表达和失调。