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蚂蚁淘在线 / 品牌中心 / Abeomics / Abeomics/MIP-2 Leeporter™ Luciferase Reporter-HEK293 Cell Line/For Profit/14-103ACL

Abeomics/MIP-2 Leeporter™ Luciferase Reporter-HEK293 Cell Line/For Profit/14-103ACL

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货号：14-103ACL
浏览量：127
品牌：Abeomics
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Amount :Non Profit

The MIP-2 Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the MIP-2 promoter. Macrophage inflammatory protein 2 (MIP-2) is a small cytokine that belongs to the C-X-C chemokine family and is also known as CXCL2. MIP-2 is one of the major proinflammatory cytokines, which is induced by innate immune receptors such TLRs and Nods, and also mediates LPS-induced osteoclastogenesis. The MIP-2 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figures 1.

Content :Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :Immediately upon receipt, store in liquid nitrogen.

Application:

  • Monitor the MIP-2 induction activity.
  • Screen for activators or inhibitors of the MIP-2 signaling pathway.

Culture conditions:

Cells should be grown at 37oC with 5%CO2using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3µg/ml of Puromycin(Note: Puromycin can be omitted during the reporter cell assays).
It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37oCwater-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in37oC-CO2incubator.
Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation.At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.
To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.To achieve satisfactory results, cells should not be passaged over 16 times.
Functional validation:
A. Response of MIP-2 Leeporter™ –HEK293 cells to phorbol 12-myristate 13-acetate (PMA).
1. Harvest MIP-2 Leeporter™ –HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100µl of growth medium at 5 x 10^4 cells/well.
2. Incubate cells at37oCin aCO2incubator for overnight.
3. The next day, stimulate cells with different concentrations of PMA.
4. Incubate at37oCin aCO2incubator for 16 hours.
5. Equilibrate the plate to room temperature for 10 minutes.
6. Add 50µl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well.
7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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