ThismousemonoclonalantibodywasgeneratedagainstplantactinACT2andrecognizessubclasses1and3ofvegetativeplantactins(arABIdopsisACT2,8,11).
ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Invertebratestherearethreegroupsofactinisoforms:alpha,betaandgamma.Thealphaactinsarefoundinmuscletissuesandareamajorconstituentofthecontractileapparatus.Thebetaandgammaactinsco-existsinmostcelltypesascomponentsofthecytoskeletonandasmediatorsofinternalcellmotility.
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
PartofTheInvestigator"sAnnexeprogram.
Specifications
Anti-Actin-2[mAb13a]Antibody
| ProductType: | Antibody |
| AccessionID: | AEE76148 |
| Antigen: | ArabidopsisActin(ACT2) |
| MolecularWeight: | 45kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| CloneName: | mAb13a |
| Reactivity: | Plantactins |
| Immunogen: | PurifiedArabidopsisactin(ACT2) |
| SpeciesImmunized: | Mouse |
| PurificationMethod: | ProteinGcolumn |
| Buffer: | 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide |
| TestedApplications: | WB(1-2ug/mL),IF |
| Storage: | -20C |
| Shipped: | Coldpacks |
Actin-2[mAb13a]Antibody
| AntigenName: | ArabidopsisActin(ACT2) |
| AccessionID: | AEE76148 |
| MolecularWeight: | 45kDa |
| ImmunoglobulinIsotype: | IgG1 |
| Clonality: | Monoclonal |
| CloneName: | mAb13a |
| SpeciesReactivity: | Plantactins |
| TypeofImmunogen: | PurifiedArabidopsisactin(ACT2) |
| SpeciesImmunized: | Mouse |
| PurificationMethod: | ProteinGcolumn |
| StorageBuffer: | 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide |
| TestedApplications: | WB(1-2ug/mL),IF |
| StorageTemperature: | -20C |
| Shipped: | Coldpacks |
FindthebestActinAntibodyforyourresearch:
| Antibody | Reactivity |
| Actin[mAbGEa] | Universal |
| Actin-1[mAb45a] | ACT1,3,4,12 |
| Actin-11[mAb2345a] | ACT1,3,4,7,11,12 |
| Actin-2[mAb13a] | ACT2,8,11 |
| Actin-8[mAbGPa] | ACT1,2,3,4,7,8,11,12 |
Data
WesternBlotAnalysis
ImmunofluorescenceStainingAnalysis
Adaptedfrom:Kandasamy,M.K.,etal.(2001)PlantCell13,1541-54.
References
- Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
- Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
- Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.
Research
| Antibody | Reactivity |
| Actin[mAbGEa] | Universal |
| Actin-1[mAb45a] | ACT1,3,4,12 |
| Actin-11[mAb2345a] | ACT1,3,4,7,11,12 |
| Actin-2[mAb13a] | ACT2,8,11 |
| Actin-8[mAbGPa] | ACT1,2,3,4,7,8,11,12 |
Data
WesternBlotAnalysis
ImmunofluorescenceStainingAnalysis
Adaptedfrom:Kandasamy,M.K.,etal.(2001)PlantCell13,1541-54.
Provider
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
References
- Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
- Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
- Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.
抗DNA-RNA杂合体[S9.6]抗体
Anti-DNA-RNA Hybrid [S9.6] Antibody
针对ΦX174噬菌体衍生的合成DNA-RNA抗原产生该小鼠单克隆抗体，并识别各种长度的RNA-DNA杂交体。
强调：
对DNA-RNA杂交体具有高度特异性和亲和力
可用于检测R环
不与单链DNA或双链DNA交叉反应
对于富含AU的双链RNA，已观察到轻微的交叉反应（约5倍）。
对于长度为8,10,15和23个碱基对的杂交体显示出高亲和力结合
DNA-RNA杂合体是真核细胞中的天然存在，这些za种的水平在具有高转录活性的位点增加，例如在转录起始，抑制和延伸期间。由于RNA-DNA杂交体影响基因组不稳定性，S9.6抗体是一种有用的试剂，可帮助研究这些za种在DNA复制或其他细胞过程中形成的R环和损伤的后果。此外，S9.6抗体可有效识别用于微阵列研究的RNA-DNA杂交。
来自国家过敏和传染病研究所/ NIH 的Stephen H. Leppla博士实验室。
C货号
品名
规格
ENH001
Anti-DNA-RNA Hybrid [S9.6] Antibody, 100ug
100ug
ENH002
Anti-DNA-RNA Hybrid [S9.6] Antibody, 1mg (10x100ug)
1mg (10x100ug)
