ThismouseIgMkmonoclonalantibodywasgeneratedagainstglycanandrecognizesgumandtomatoacetylatedmannan.
Highlights:
- Reactswithgumandtomatoacetylatedmannan
- SuitableforELISA,ImmunolabelingandImmunofluorescenceapplications
Mannanisoneofthemajorconstituentgroupsofhemicelluloseinthewallofhigherplants.ItcompriseslinearorbranchedpolymersderivedfromsugarssuchasD-mannose,D-galactose,andD-glucose.
FromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.
Specifications
Anti-AcetylatedMannan[CCRC-M169,2C2.D8.E5]Antibody(supernatant)
ProductType: | Antibody |
Antigen: | AcetylatedMannan |
AccessionID: | CCRCM169 |
Isotype: | IgMk |
Clonality: | Monoclonal |
CloneName: | CCRCM169:2C2.D8.E5 |
Reactivity: | Gumandtomato |
Immunogen: | Pectin/MeBSA |
SpeciesImmunized: | Mouse |
Buffer: | Cellculturesupernatant |
TestedApplications: | ELISA,Immunolabeling,IF |
Storage: | <1 month="" at="" 4c,="">1monthat-80C1> |
Shipped: | Dryice |
Documentation
Notes:
Strongreactivitytowardtomatoglucomannan.Weaktomoderatereactivitytoward(1?3)(1?4)-ß-glucan.
ThesemonoclonalantibodiesweredevelopedunderthesponsorshipoftheUSNationalScienceFoundation,throughawardnumberDBI-0421683.Theiruseinbiomasscharacterization,studyofbiomassdeconstructionandquantitationwasdevelopedunderthesponsorshipoftheUSDepartmentofEnergythroughawardsDE-PS02-06ER64304andDE-AC05-00OR22725(BioEenergyScienceCenter).
AntigenName: | AcetylatedMannan |
AccessionID: | CCRCM169 |
ImmunoglobinIsotype: | IgMk |
Clonality: | Monoclonal |
CloneName: | CCRCM169:2C2.D8.E5 |
Immunogen: | Pectin/MeBSA |
SpeciesReactivity: | Gumandtomato |
SpeciesImmunized: | Mouse |
FinalBufferConcentration: | Cellculturesupernatant |
TestedApplications: | ELISA,Immunolabeling,IF |
StorageTemperature: | <1monthat4C,>1monthat-80C |
Shipped: | DryIce |
Notes:
Strongreactivitytowardtomatoglucomannan.Weaktomoderatereactivitytoward(1→3)(1→4)-β-glucan.
ThesemonoclonalantibodiesweredevelopedunderthesponsorshipoftheUSNationalScienceFoundation,throughawardnumberDBI-0421683.Theiruseinbiomasscharacterization,studyofbiomassdeconstructionandquantitationwasdevelopedunderthesponsorshipoftheUSDepartmentofEnergythroughawardsDE-PS02-06ER64304andDE-AC05-00OR22725(BioEenergyScienceCenter).
References
- RobinE.Young,HeatherE.McFarlane,MichaelG.Hahn,TamaraL.Western,GeorgeW.HaughnandA.LaceySamuels.2008.AnalysisoftheGolgiApparatusinArABIdopsisSeedCoatCellsduringPolarizedSecretionofPectin-RichMucilage.ThePlantCellJune2008vol.20no.61623-1638.
- DeMartini,JD,Pattathil,S,Avci,U,Szekalski,K,Mazumder,K,Hahn,M.G.,Wyman,CE:Applicationofmonoclonalantibodiestoinvestigateplantcellwalldeconstructionforbiofuelsproduction.EnergyEnviron.Sci.,2011,4,4332-4339.
- PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
- A.P.deSouza,D.C.C.Leite,S.Pattahil,M.G.Hahn,M.S.Buckeridge.2013.Compositionandstructureofsugarcanecellwallpolysaccharides:Implicationsforsecondgenerationbioethanolproduction.BioenergyResearch6:564-579.
- J.Puhlmann,E.Bucheli,M.J.Swain,N.Dunning,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1994)Generationofmonoclonalantibodiesagainstplantcellwallpolysaccharides.I.Characterizationofamonoclonalantibodytoaterminalalpha-(1,2)-linkedfucosyl-containingepitope.PlantPhysiol.104:699-710.
- G.Freshour,R.P.Clay,M.S.Fuller,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1996)Developmentalandtissue-specificstructuralalterationsofthecell-wallpolysaccharidesofArabidopsisthalianaroots.PlantPhysiol.110:1413-1429.
- G.Freshour,C.P.Bonin,W.-D.Reiter,P.Albersheim,A.G.Darvill,andM.G.Hahn.(2003)Distributionoffucose-containingxyloglucansincellwallsofthemur1mutantofArabidopsisthaliana.PlantPhysiol.131:1602-1612.
- PattathilS,AvciU,BaldwinD,SwennesAG,McGillJA,PopperZ,BoottenT,AlbertA,DavisRH,ChennareddyC,DongR,O"SheaB,RossiR,LeoffC,FreshourG,NarraR,O"NeilM,YorkWS,HahnMG.(2010)AComprehensiveToolkitofPlantCellWallGlycan-DirectedMonoclonalAntibodies.PlantPhysiol.153:514-525.
- PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
- PattathilS,AvciU,BaldwinD,etal.2010.Acomprehensivetoolkitofplantcellwallglycan-directedmonoclonalantibodies.PlantPhysiology153,514-525.
- PattathilS,HahnMG,DaleBE,ChundawatSP.Insightsintoplantcellwallstructure,architecture,andintegrityusingglycomeprofilingofnativeandAFEXTM-pre-treatedbiomass.JExpBot.2015Jul;66(14):4279-94.
Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.
Provider
FromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.
References
- RobinE.Young,HeatherE.McFarlane,MichaelG.Hahn,TamaraL.Western,GeorgeW.HaughnandA.LaceySamuels.2008.AnalysisoftheGolgiApparatusinArabidopsisSeedCoatCellsduringPolarizedSecretionofPectin-RichMucilage.ThePlantCellJune2008vol.20no.61623-1638.
- DeMartini,JD,Pattathil,S,Avci,U,Szekalski,K,Mazumder,K,Hahn,M.G.,Wyman,CE:Applicationofmonoclonalantibodiestoinvestigateplantcellwalldeconstructionforbiofuelsproduction.EnergyEnviron.Sci.,2011,4,4332-4339.
- PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
- A.P.deSouza,D.C.C.Leite,S.Pattahil,M.G.Hahn,M.S.Buckeridge.2013.Compositionandstructureofsugarcanecellwallpolysaccharides:Implicationsforsecondgenerationbioethanolproduction.BioenergyResearch6:564-579.
- J.Puhlmann,E.Bucheli,M.J.Swain,N.Dunning,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1994)Generationofmonoclonalantibodiesagainstplantcellwallpolysaccharides.I.Characterizationofamonoclonalantibodytoaterminalalpha-(1,2)-linkedfucosyl-containingepitope.PlantPhysiol.104:699-710.
- G.Freshour,R.P.Clay,M.S.Fuller,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1996)Developmentalandtissue-specificstructuralalterationsofthecell-wallpolysaccharidesofArabidopsisthalianaroots.PlantPhysiol.110:1413-1429.
- G.Freshour,C.P.Bonin,W.-D.Reiter,P.Albersheim,A.G.Darvill,andM.G.Hahn.(2003)Distributionoffucose-containingxyloglucansincellwallsofthemur1mutantofArabidopsisthaliana.PlantPhysiol.131:1602-1612.
- PattathilS,AvciU,BaldwinD,SwennesAG,McGillJA,PopperZ,BoottenT,AlbertA,DavisRH,ChennareddyC,DongR,O"SheaB,RossiR,LeoffC,FreshourG,NarraR,O"NeilM,YorkWS,HahnMG.(2010)AComprehensiveToolkitofPlantCellWallGlycan-DirectedMonoclonalAntibodies.PlantPhysiol.153:514-525.
- PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
- PattathilS,AvciU,BaldwinD,etal.2010.Acomprehensivetoolkitofplantcellwallglycan-directedmonoclonalantibodies.PlantPhysiology153,514-525.
- PattathilS,HahnMG,DaleBE,ChundawatSP.Insightsintoplantcellwallstructure,architecture,andintegrityusingglycomeprofilingofnativeandAFEXTM-pre-treatedbiomass.JExpBot.2015Jul;66(14):4279-94.
Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.
抗DNA-RNA杂合体[S9.6]抗体
Anti-DNA-RNA Hybrid [S9.6] Antibody
针对ΦX174噬菌体衍生的合成DNA-RNA抗原产生该小鼠单克隆抗体,并识别各种长度的RNA-DNA杂交体。
强调:
对DNA-RNA杂交体具有高度特异性和亲和力
可用于检测R环
不与单链DNA或双链DNA交叉反应
对于富含AU的双链RNA,已观察到轻微的交叉反应(约5倍)。
对于长度为8,10,15和23个碱基对的杂交体显示出高亲和力结合
DNA-RNA杂合体是真核细胞中的天然存在,这些za种的水平在具有高转录活性的位点增加,例如在转录起始,抑制和延伸期间。由于RNA-DNA杂交体影响基因组不稳定性,S9.6抗体是一种有用的试剂,可帮助研究这些za种在DNA复制或其他细胞过程中形成的R环和损伤的后果。此外,S9.6抗体可有效识别用于微阵列研究的RNA-DNA杂交。
来自国家过敏和传染病研究所/ NIH 的Stephen H. Leppla博士实验室。
C货号
品名
规格
ENH001
Anti-DNA-RNA Hybrid [S9.6] Antibody, 100ug
100ug
ENH002
Anti-DNA-RNA Hybrid [S9.6] Antibody, 1mg (10x100ug)
1mg (10x100ug)